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    Vector Laboratories vector laboratories ref
    Vector Laboratories Ref, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 715 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 6. VSCTs are glutamatergic, possess spinal axon collaterals, and make synapses with Chx10 spinal neurons (A) (A1–3) VSCTs (CTb-488 from cerebellum, green) and VGluT2 mRNA (red) and merged image. Inset: VSCT (arrow in A1–3) expressing VGluT2. (B) Percentage of VSCTs expressing VGluT2 (N = 3). (C) A P4 L2 VSCT filled with <t>neurobiotin</t> (red) after intracellular recording co-localizing with CTb-488 (cerebellum at P0). MNs were backfilled with a dextran dye from the VR (blue). White arrows mark VSCT axon. (D) Higher mag of VSCT (in C), showing its main axon (white arrow) and an ipsilaterally projecting axon collateral (inset, double arrow; magnified from the dotted box). (E) AAV2/1-Flex-nGFP-N2c-Gp-TVA injected in spinal cord at P0 to introduce the rabies G protein to Chx10 neurons (Chx10Cre mice). Concurrently, CTb- 647 injected in cerebellum to mark VSCTs. Rabies- N2c-EnvA-dsRed was injected in spinal cord at P10. (F) The ‘‘starter’’ Chx10 neurons are labeled with nuclear GFP and cytoplasmic dsRed. Neurons providing monosynaptic input to ‘‘starter’’ neurons are labeled with dsRed but not nGFP. VSCTs are identified through CTb-647. (G) A Chx10 ‘‘starter’’ neuron identified by co- localization of dsRed and nGFP (insets). Arrows indicate VSCT axon collaterals synapsing onto the Chx10 ‘‘starter’’ neuron. (H) VSCT (yellow arrowhead in insets) labeled by transynaptic transfection of its axon collaterals onto the Chx10 neuron. Data are represented as mean ± SEM. See also Figures S6 and S7.
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    Figure 6. VSCTs are glutamatergic, possess spinal axon collaterals, and make synapses with Chx10 spinal neurons (A) (A1–3) VSCTs (CTb-488 from cerebellum, green) and VGluT2 mRNA (red) and merged image. Inset: VSCT (arrow in A1–3) expressing VGluT2. (B) Percentage of VSCTs expressing VGluT2 (N = 3). (C) A P4 L2 VSCT filled with <t>neurobiotin</t> (red) after intracellular recording co-localizing with CTb-488 (cerebellum at P0). MNs were backfilled with a dextran dye from the VR (blue). White arrows mark VSCT axon. (D) Higher mag of VSCT (in C), showing its main axon (white arrow) and an ipsilaterally projecting axon collateral (inset, double arrow; magnified from the dotted box). (E) AAV2/1-Flex-nGFP-N2c-Gp-TVA injected in spinal cord at P0 to introduce the rabies G protein to Chx10 neurons (Chx10Cre mice). Concurrently, CTb- 647 injected in cerebellum to mark VSCTs. Rabies- N2c-EnvA-dsRed was injected in spinal cord at P10. (F) The ‘‘starter’’ Chx10 neurons are labeled with nuclear GFP and cytoplasmic dsRed. Neurons providing monosynaptic input to ‘‘starter’’ neurons are labeled with dsRed but not nGFP. VSCTs are identified through CTb-647. (G) A Chx10 ‘‘starter’’ neuron identified by co- localization of dsRed and nGFP (insets). Arrows indicate VSCT axon collaterals synapsing onto the Chx10 ‘‘starter’’ neuron. (H) VSCT (yellow arrowhead in insets) labeled by transynaptic transfection of its axon collaterals onto the Chx10 neuron. Data are represented as mean ± SEM. See also Figures S6 and S7.
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    Figure 6. VSCTs are glutamatergic, possess spinal axon collaterals, and make synapses with Chx10 spinal neurons (A) (A1–3) VSCTs (CTb-488 from cerebellum, green) and VGluT2 mRNA (red) and merged image. Inset: VSCT (arrow in A1–3) expressing VGluT2. (B) Percentage of VSCTs expressing VGluT2 (N = 3). (C) A P4 L2 VSCT filled with <t>neurobiotin</t> (red) after intracellular recording co-localizing with CTb-488 (cerebellum at P0). MNs were backfilled with a dextran dye from the VR (blue). White arrows mark VSCT axon. (D) Higher mag of VSCT (in C), showing its main axon (white arrow) and an ipsilaterally projecting axon collateral (inset, double arrow; magnified from the dotted box). (E) AAV2/1-Flex-nGFP-N2c-Gp-TVA injected in spinal cord at P0 to introduce the rabies G protein to Chx10 neurons (Chx10Cre mice). Concurrently, CTb- 647 injected in cerebellum to mark VSCTs. Rabies- N2c-EnvA-dsRed was injected in spinal cord at P10. (F) The ‘‘starter’’ Chx10 neurons are labeled with nuclear GFP and cytoplasmic dsRed. Neurons providing monosynaptic input to ‘‘starter’’ neurons are labeled with dsRed but not nGFP. VSCTs are identified through CTb-647. (G) A Chx10 ‘‘starter’’ neuron identified by co- localization of dsRed and nGFP (insets). Arrows indicate VSCT axon collaterals synapsing onto the Chx10 ‘‘starter’’ neuron. (H) VSCT (yellow arrowhead in insets) labeled by transynaptic transfection of its axon collaterals onto the Chx10 neuron. Data are represented as mean ± SEM. See also Figures S6 and S7.
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    Figure 6. VSCTs are glutamatergic, possess spinal axon collaterals, and make synapses with Chx10 spinal neurons (A) (A1–3) VSCTs (CTb-488 from cerebellum, green) and VGluT2 mRNA (red) and merged image. Inset: VSCT (arrow in A1–3) expressing VGluT2. (B) Percentage of VSCTs expressing VGluT2 (N = 3). (C) A P4 L2 VSCT filled with <t>neurobiotin</t> (red) after intracellular recording co-localizing with CTb-488 (cerebellum at P0). MNs were backfilled with a dextran dye from the VR (blue). White arrows mark VSCT axon. (D) Higher mag of VSCT (in C), showing its main axon (white arrow) and an ipsilaterally projecting axon collateral (inset, double arrow; magnified from the dotted box). (E) AAV2/1-Flex-nGFP-N2c-Gp-TVA injected in spinal cord at P0 to introduce the rabies G protein to Chx10 neurons (Chx10Cre mice). Concurrently, CTb- 647 injected in cerebellum to mark VSCTs. Rabies- N2c-EnvA-dsRed was injected in spinal cord at P10. (F) The ‘‘starter’’ Chx10 neurons are labeled with nuclear GFP and cytoplasmic dsRed. Neurons providing monosynaptic input to ‘‘starter’’ neurons are labeled with dsRed but not nGFP. VSCTs are identified through CTb-647. (G) A Chx10 ‘‘starter’’ neuron identified by co- localization of dsRed and nGFP (insets). Arrows indicate VSCT axon collaterals synapsing onto the Chx10 ‘‘starter’’ neuron. (H) VSCT (yellow arrowhead in insets) labeled by transynaptic transfection of its axon collaterals onto the Chx10 neuron. Data are represented as mean ± SEM. See also Figures S6 and S7.
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    Image Search Results


    Figure 6. VSCTs are glutamatergic, possess spinal axon collaterals, and make synapses with Chx10 spinal neurons (A) (A1–3) VSCTs (CTb-488 from cerebellum, green) and VGluT2 mRNA (red) and merged image. Inset: VSCT (arrow in A1–3) expressing VGluT2. (B) Percentage of VSCTs expressing VGluT2 (N = 3). (C) A P4 L2 VSCT filled with neurobiotin (red) after intracellular recording co-localizing with CTb-488 (cerebellum at P0). MNs were backfilled with a dextran dye from the VR (blue). White arrows mark VSCT axon. (D) Higher mag of VSCT (in C), showing its main axon (white arrow) and an ipsilaterally projecting axon collateral (inset, double arrow; magnified from the dotted box). (E) AAV2/1-Flex-nGFP-N2c-Gp-TVA injected in spinal cord at P0 to introduce the rabies G protein to Chx10 neurons (Chx10Cre mice). Concurrently, CTb- 647 injected in cerebellum to mark VSCTs. Rabies- N2c-EnvA-dsRed was injected in spinal cord at P10. (F) The ‘‘starter’’ Chx10 neurons are labeled with nuclear GFP and cytoplasmic dsRed. Neurons providing monosynaptic input to ‘‘starter’’ neurons are labeled with dsRed but not nGFP. VSCTs are identified through CTb-647. (G) A Chx10 ‘‘starter’’ neuron identified by co- localization of dsRed and nGFP (insets). Arrows indicate VSCT axon collaterals synapsing onto the Chx10 ‘‘starter’’ neuron. (H) VSCT (yellow arrowhead in insets) labeled by transynaptic transfection of its axon collaterals onto the Chx10 neuron. Data are represented as mean ± SEM. See also Figures S6 and S7.

    Journal: Cell

    Article Title: Control of mammalian locomotion by ventral spinocerebellar tract neurons.

    doi: 10.1016/j.cell.2021.12.014

    Figure Lengend Snippet: Figure 6. VSCTs are glutamatergic, possess spinal axon collaterals, and make synapses with Chx10 spinal neurons (A) (A1–3) VSCTs (CTb-488 from cerebellum, green) and VGluT2 mRNA (red) and merged image. Inset: VSCT (arrow in A1–3) expressing VGluT2. (B) Percentage of VSCTs expressing VGluT2 (N = 3). (C) A P4 L2 VSCT filled with neurobiotin (red) after intracellular recording co-localizing with CTb-488 (cerebellum at P0). MNs were backfilled with a dextran dye from the VR (blue). White arrows mark VSCT axon. (D) Higher mag of VSCT (in C), showing its main axon (white arrow) and an ipsilaterally projecting axon collateral (inset, double arrow; magnified from the dotted box). (E) AAV2/1-Flex-nGFP-N2c-Gp-TVA injected in spinal cord at P0 to introduce the rabies G protein to Chx10 neurons (Chx10Cre mice). Concurrently, CTb- 647 injected in cerebellum to mark VSCTs. Rabies- N2c-EnvA-dsRed was injected in spinal cord at P10. (F) The ‘‘starter’’ Chx10 neurons are labeled with nuclear GFP and cytoplasmic dsRed. Neurons providing monosynaptic input to ‘‘starter’’ neurons are labeled with dsRed but not nGFP. VSCTs are identified through CTb-647. (G) A Chx10 ‘‘starter’’ neuron identified by co- localization of dsRed and nGFP (insets). Arrows indicate VSCT axon collaterals synapsing onto the Chx10 ‘‘starter’’ neuron. (H) VSCT (yellow arrowhead in insets) labeled by transynaptic transfection of its axon collaterals onto the Chx10 neuron. Data are represented as mean ± SEM. See also Figures S6 and S7.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mecamylamine hydrochloride Tocris CAT# 2843 NBQX disodium salt Tocris CAT# 1044 NEUROBIOTIN Vector Laboratories REF# SP-1120 NMDA Tocris CAT# 0114 Serotonin hydrochloride Tocris CAT# 3547 Texas Red Dextran Invitrogen CAT# D1828 ZD 7288 Tocris CAT# 1000 Critical commercial assays HNPP Fluorescent Detection Set Roche CAT# 11758888001 Experimental models: Organisms/strains Mouse: C57BL/6J JAX #000664; RRID:IMSR_JAX:000664 Mouse: B6.Cg-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J JAX #024109; RRID:IMSR_JAX:024109 Mouse: B6.129-Gt(ROSA)26Sortm1(CAG-CHRM4*,-mCitrine)Ute/J JAX #026219; RRID:IMSR_JAX:026219 Mouse: B6.Cg-Gt(ROSA)26Sortm40.1(CAG-aop3/EGFP)Hze/J JAX #021188; RRID:IMSR_JAX:021188 Mouse: B6;129S6-Gt(ROSA)26Sortm9(CAG-mCherry,-CHRM4*)Dym/J JAX #029040; RRID:IMSR_JAX:029040 Mouse: FVB/N-Tg(Cdx2-flpo)2Ddg/J JAX #030288; RRID:IMSR_JAX:030288 Mouse: Chx10-CRE Thomas Jessell Lab (Crone et al., 2008) N/A Oligonucleotides Primers for B6.Cg-Gt(ROSA)26Sortm32(CAG-COP4*H134R/ EYFP)Hze/J.

    Techniques: Expressing, Injection, Introduce, Labeling, Transfection